Different pH levels medium effects in IAA production of phylloplane bacterium Serratia plymuthica strain UBCF_13

Jamsari, Jamsari Different pH levels medium effects in IAA production of phylloplane bacterium Serratia plymuthica strain UBCF_13. IOP Proceeding.

Preview

Text Wandira_2021_IOP_Conf._Ser.__Earth_Environ._Sci._741_012003.pdf Download (1MB) | Preview

Abstract

Indole-3-acetic acid [IAA] is the main source of auxin in plants that plays a major role in controlling various physiological processes. The ability of bacterium in IAA biosynthesis depends on several environmental factors, usually triggered by acidic stresses. This study was aimed to optimize the IAA production by phylloplane bacterium Serratiaplymuthica strain UBCF_13 with various pH levels. Optimization was carried out by using 0.2% L-Tryptophan in Luria Bertani[LB] media with various pH levels namely pH 4.0; 4.5; 5.0; 5.5; 6.0; 6.5; 7.0 and 9.0. UBCF_13 showed the best IAA production at pH 6.0 and 0.2% L-Tryptophan exhibited IAA concentration in the amount of 24.13 ppm. The UBCF_13 extracellular protein profile analysis showed that at pH 6.0 induced L-Tryptophan 0.2% produced protein bands of 60.8 kDa and 45 kDa in size. These protein bands are assumed to be ipdC and nitrilase proteins which are involved in the IAA biosynthesis. The application of IAA was carried out using chili peppers [Capsicum annuum L.] germination of the Lotanbar genotype. The application was using supernatant produced from media of pH 6.0 and 0.2% LTryptophan. The results show that root and hypocotyl lengths were 4.10 cm and 2.90 cm, respectively. Meanwhile, root and hypocotyl lengths in the control sample only 2.78 cm and 1.88 cm. The results indicated that the IAA production of UBCF_13 can promote the growth of chili seedling. A further detailed study on the visualization of protein profiles at the optimum treatment needs to be done. Two-DimensionalElectrophoresis is recommended to be applied so that protein bands can be accurately separated to identify detailed proteins involved in the IAA biosynthesis.

Actions (login required)

View Item