Studi In Silico, Sintesis, dan Uji Sitotoksik Senyawa P-Metoksi Kalkon terhadap Sel Kanker Payudara MCF-7

Dona, Rahma and Frimayanti, Neni and Ikhtiarudin, Ihsan and Iskandar, Benni and Maulana, Fikri and Silalahi, Nova Tantri (2019) Studi In Silico, Sintesis, dan Uji Sitotoksik Senyawa P-Metoksi Kalkon terhadap Sel Kanker Payudara MCF-7. Jurnal Sains Farmasi & Klinis, 6 (3). pp. 243-249. ISSN 2407-7062

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Abstract

Chalcone (1,3-diphenyl-2-propene-1-on) is one of the flavonoid compounds with variety of biological activities such as anticancer. the aims of this study are to determine the cytotoxic activity of chalcone analogues using molecular docking, this chalcone analogue has been synthesized using Claisen-Schmidt condensation reaction with alkaline catalyst with microwave irradiation method. In this study, chalcone analogue compound docked into proteins target with PDB ID P521, molecular docking was performed usingAutoDock Vina program. The cytotoxicity assay of a chalcone analogue was performed against breast cancer MCF-7 cell line using WST-8 Assay. Based on the docking result, chalcone analogue (E)-3 against MCF-7 breast cancer cells line because this compound has a lower bond free energy than doxorubicin as a positive control; in addition, this compound also has four amino acid residues which are the same as doxorubicin; there are four interaction between amino acid acid residues with this chalcone, they are hydrogen bond, van der Waals interaction, pi-sigma and also pi alkil. such as. Based on cytotoxicity tests, this compound have strong cytotoxic activity with IC50 of 48,18 µg/mL. The result indicated that chalcone analogue (E)-3-(4- metoksifenil)-1-fenilprop-2-en-1-on have potent as anticancer inhibitor against on MCF-7 human breast cancer cells

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