Fenolik Total, Kandungan Flavonoid, dan Aktivitas Antioksidan Ekstrak Etanol Daun Mareme (Glochidion arborescense Blume.)

Indra, Indra and Nurmalasari, Novianti and Kusmiati, Meti (2019) Fenolik Total, Kandungan Flavonoid, dan Aktivitas Antioksidan Ekstrak Etanol Daun Mareme (Glochidion arborescense Blume.). Jurnal Sains Farmasi & Klinis, 6 (3). pp. 206-212. ISSN 2407-7062

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Abstract

Glochidion arborescens Blume is often consumed by the people, especially the Sundanese as a vegetable dish. Several studies have shown that the genus Glochidion has potential as a medicinal plant. This research aimed to determine the total phenolic content, total flavonoid and antioxidant activity of Glochidion arborescens leaves. A dry powder of Glochidion arborescens leaves extracted by reflux process using ethanol-water 70% v/v. A total phenolic essay is determined by the method of Folin-Chiocalteu reagent using gallic acid as a reference. AlCl3 reagent is used to analyse the flavonoid content by comparing with quercetin. The antioxidant activity of the extract was determined by radical scavenging assay using DPPH (2,2-diphenyl-1- picrylhydrazyl) radical. Phytochemical screening of Glochidion arborescens leaves indicated the presence of flavonoids, tannins, polyphenols, monoterpenoids-sesquiterpenes, saponins, and quinones. The total phenolic content of ethanol extract of Glochidion arborescens leaves at 33.32 ± 0.25 mg Gallic Acid Equivalent (GAE)/g extract and total flavonoid content at 3.02 ± 0.003 mg quercetin equivalent (QE)/g extract. Antioxidant activity by DPPH method obtained IC50 ethanol extract of Glochidion arborescens leaves of 5.5 µg/mL, and IC50 value of vitamin C was 3.34 µg / mL. Based on these results Glochidion arborescens leaves have the potential to be developed into medicinal plants to prevent or treat diseases caused by oxidative stress

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