Isolation and characterization of the NPR1 gene from highly susceptible pepper cv. Berangkai.

Jamsari, Jamsari (2019) Isolation and characterization of the NPR1 gene from highly susceptible pepper cv. Berangkai. Bioscience Research, 16 (2). pp. 1317-1322. ISSN 2218-3973

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Abstract

Nonexpressor of pathogenesis-related-1 (NPR1) is one of the important proteins involved in plant defense systems. This protein activates systemic acquired resistance (SAR) and induces expression of Pathogenesis-related (PR) gene. In this article, the NPR1 gene has been isolated from Capsicum annum cv. Berangkai which are susceptible to infection of geminivirus. The putative full gene NPR1 Berangkai sequence, abbreviated CaNPR1.Berangkai, was obtained by assembling the sequencing results of 3 exons of the NPR1 gene. The putative full gene CaNPR1.Berangkai has a nucleotide length of 2,786 base pairs and 390 amino acids. The results of protein domain analysis show that CbNPR1 has a BTB domain at residues 60-130, ankyrin domain at residues 245-344, and NLS domains at residues 345 – 390. It is indicated that CaNPR1.Berangkai is similar to CaNPR1 Zunla and AtNPR1.

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