Mahata, maria endo and Shinya, Shoko and Masaki, Eiko and Yamamoto, Takashi and Ohnuma, Takayuki and Brzezinski, Ryszard and Mazumder, Tapan and Yamashita, Kazuhiko and Narihiro, Kazue and Fukamizo, Tamo (2014) production of chitooligosaccharides from Rhizopus oligosporus NRRL2710 cells by chitosanase digestion. Carbohydrate Research. pp. 27-33.
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Abstract
The intact cells of Rhizopus oligosporus NRRL2710, whose cell walls are abundant source of N-acetylglu- cosamine (GlcNAc) and glucosamine (GlcN), were digested with three chitinolytic enzymes, a GH-46 chitosanase from Streptomyces sp. N174 (CsnN174), a chitinase from Pyrococcus furiosus, and a chitinase from Trichoderma viride, respectively. Solubilization of the intact cells by CsnN174 was found to be the most efficient from solid state CP/MAS 13C NMR spectroscopy. Chitosanase products from Rhizopus cells were purified by cation exchange chromatography on CM-Sephadex C-25 and gel-filtration on Cellulofine Gcl-25 m. NMR and MALDI-TOF-MS analyses of the purified products revealed that GlcN–GlcNAc, (GlcN)2–GlcNAc, and (GlcN)2 were produced by the enzymatic digestion of the intact cells. The chitosan- ase digestion of Rhizopus cells was found to be an excellent system for the conversion of fungal biomass without any environmental impact.
| Item Type: | Article |
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| Subjects: | S Agriculture > SF Animal culture |
| Divisions: | Fakultas Peternakan |
| Depositing User: | Maria Mahata |
| Date Deposited: | 04 Feb 2019 08:28 |
| Last Modified: | 04 Feb 2019 08:28 |
| URI: | http://repo.unand.ac.id/id/eprint/21600 |
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