Decreased Interleukin-4 Level of Type I Hypersensitive Mice Using Scopoloetin Isolated from Noni Fruit (Morinda citrifolia L.)

Aldi, Yufri and Yuliandra, Yori and Nasrul, Ellyza and Yanwirasti, Yanwirasti and Handayani, Dian and Bakhtiar, Amri Decreased Interleukin-4 Level of Type I Hypersensitive Mice Using Scopoloetin Isolated from Noni Fruit (Morinda citrifolia L.). Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6 (4). pp. 1823-1829. ISSN 0975-8585

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Abstract

An in vivo study of the activity of scopoletin isolated from noni fruit (Morinda citrifolia L.) on the level of interleukin-4 (IL-4) in type I hypersensitive male Swiss-Webster mice has been carried out. Scopoletin was isolated from dried noni powder by soxhletation method using dichloromethane, separated by column chromatography using silica gel as stationary phase and n-hexane-ethyl acetate (1:4) as mobile phase, then purified by column chromatography using Sephadex LH20 as stationary phase and methanol as mobile phase. Type I hypersensitive male mice were obtained by ovalbumin sensitization. Animal model were divided into 5 groups: negative control group, positive control group, and scopoletin-treated group (1; 3; and 10 mg/kg). The results showed that scopoletin at doses of 1, 3 and 10 mg/kg decreased the level of IL-4 of type I hypersensitive mice significantly (p <0.01). The scopoletin at the dose of 10 mg/kg decreased the serum level of IL-4 to the normal level.

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