Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.) from West Sumatera

Jamsari, JMS (2017) Optimal Media for In-Vitro Regeneration of Two Local Genotypes of Chili Pepper (Capsicum annuum L.) from West Sumatera. IJASET, 7 (3). pp. 904-909. ISSN 2088-5334

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Abstract

Genetic engineering strategy in chilli peppers (Capsicum annuum L.) has been applied so far to improve its genetic capacity for instance against some important diseases. This strategy needs an established system for calli preparation as well as transformant regeneration. In most cases, this necessity is genotype dependent especially for some local genotypes. Based on this, we optimized media for calli induction, shoot and root regeneration of our three local cultivars namely Kampung, Kopay, and Trisula. Media combinations basically were composed of MS and combined with some growth factors, for instance, IAA, BAP, NAA and TDZ with different combinations for each calli induction, shoot and root regeneration. All media compositions were supplemented with 30 g/L sucroses. The best medium for calli induction was MS media supplemented with 4 mg/L BAP, 0.5 mg/L IAA and L2 vitamin (Thiamin-HCl and Pyrodoxine-HCl). While the best medium for shoot induction and elongation was MS supplemented with 1mg/L BAP, 5 mg/L AgNO3, 2 mg/L GA3, 2 mg/L Calcium Pantothenate and L2 vitamin. Furthermore, the best medium for root induction and elongation was WPM (Woody Plant Medium) supplemented with 0.4 mg/L NAA. This finding should provide optimal media for calli induction and shoot-root regeneration for the three local cultivars Kampung, Kopay, and Trisula.

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