Anggraini, L and Marlida, Y and Wizna, W and Jamsari, J and Mirzah, M
(2019)
Optimization of Nutrient Medium for Pediococcus acidilactici DS15
to Produce GABA.
Journal of World's Poultry Research, 9 (3).
pp. 139-146.
ISSN 2322-455X
Abstract
Nutrition is an essential factor for microorganisms to grow and survive. Carbon and nitrogen sources are used in
producing primary and secondary metabolites. Gamma-Aminobutyric acid (GABA), a non-coded amino acid, is a
secondary metabolite which acts as an inhibitory neurotransmitter of the central nervous system. Pediococcus
acidilactici DS15 is a bacterium belonging to the order of Lactic Acid Bacteria. This study aimed to determine the
effects of nutrients including glutamate, nitrogen and carbon sources on GABA production by Pediococcus
acidilactici DS15. The tests were carried out using a range of 30 mM, 40 mM, 50 mM, 60 mM, and 70 mM glutamate
as inducer and carbon sources in the form of peptone, yeast extract, skim milk, NH4NO3, KNO3, whey tofu and soy
milk as nitrogen sources, and then glucose, lactose, maltosa, sucrose, palm, and cane sugar as carbon sources. The
best sources of both will be tested with levels 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100% for nitrogen and 1, 3, 5, 7,
9, 11, 13 and 15% for carbon. The results of this investigation revealed that the addition of 60 mM glutamate caused
the higher amount of GABA production and the best source of nitrogen and carbon for Pediococcus acidilactici DS15
were 100% whey tofu and 15% palm sugar, respectively. Production rate of GABA by Pediococcus acidilactici DS15
could reach up to 311,485 mg / L.
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Optimization of Nutrient Medium for Pediococcus acidilactici DS15
to Produce GABA. (deposited 08 Feb 2021 01:14)
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