Pengaruh Diabetes Terhadap Haemodinamik dan Aktifitas Saraf Simpatik Ginjal pada Tikus Sprague-Dawley

Helmi, A. and Armenia, Armenia and Jhons, EL and Munawar, AL and Yusof, APM (2010) Pengaruh Diabetes Terhadap Haemodinamik dan Aktifitas Saraf Simpatik Ginjal pada Tikus Sprague-Dawley. Jurnal Sains dan Teknologi Farmasi.

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Abstract

This study sets up to examine the effect of diabetes on the haemodinamic and renal sympathetic nerve activity in normal male Sprague-Dawley (SD) rats. Diabetes was chemically induced using streptozotocin (STZ) 60 mg/kg intraperitoneally, and the experiment was caried out seven days later. Body weight, total water intake, total urine output, urine sodium excretion were determined every 24 hours after STZ. The blood glucose, systolic and diastolic blood pressure (SBP and DBP), heaft rate (HR) and renal sympathetic nerve activity (RSNA) were measured the day of acut experiment conducted. The animals were anaesthetised by a mixture of fluothane/O2 with flow rate 4 and 5 l/min for 2 min and thereafter maintained on chloralose/urethane (12 mg and 180 mg/ml) 0.1 ml/5 min (6-7 times) followed by 0.05 ml/30 min respectively, administered intravenously via femoral vein. Blood pressure (systolic and diastolic) and heart rate were monitored using a Grass transducer connected to LabVIEW data acquisition system on a Macintosh computer. Renal nerves were isolated and placed on bipolar elechodes and their activity measured using the above system. Blood pressure, heart rate and renal sympathetic nerve activity were measured. The same experiment was also done in the normal SD rats. Results showed that urine out flow, water intack, natrium urine and blood glucose levels were increased in diabetic state while body weight was redused. Blood pressure were increurq but HR was decrease. However on RSNA was increased in diabetic state. Collectivily, these data indicated that diabetes can influence blood pressure, heart rate, renal sympathetic nerve activity in experimental diabetes rats.

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