Production and Characterization of Microbial Polyesters Poly (3-Hydroxybutyrat) From Oleic Acid as Sole Carbon Source

Djamaan, Akmal and Fitriani, Lili and wangi, QA and ismed, Friardi and Zaini, Erizal (2015) Production and Characterization of Microbial Polyesters Poly (3-Hydroxybutyrat) From Oleic Acid as Sole Carbon Source. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6 (1). pp. 814-822. ISSN 0975-8585

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Abstract

Production and characterization of microbial polyesters poly(3-hydroxybutyrate), P(3HB) from oleic acid as sole carbon source by Erwinia sp. USMI-20 have been carried out. The polymer granules accumulated in the cells of Erwinia sp. USMI-20 upon cultivation were identified by transmission electron microscopy (TEM). P(3HB) was extracted and characterized by using 1H and 13C nuclear magnetic resonance (NMR). The thermal properties including melting temperature (Tm) and glass transition temperature (Tg) for the polymer were analyzed by differential scanning calorimetry (DSC). The weigh-average molecular weight (Mw), number-average molecular weight (Mn) and polydispersity index (Mw/Mn) were determined by gel permeation chromatography (GPC). It was found that Erwinia sp. USMI-20 could produced P(3HB) with a maximum polymer content 55 % of the dry cell weight, an amount of polymer 1.9 g/l, a dry cell weight 3.5 g/l, a maximum specific growth rate at 0.30 /h, a maximum polymer production rate at 0.03/h, and YP(3HB)/C 0.39 g/g , with the optimum fermentation time of 54 hours. The Tm and Tg of P(3HB) were 157 oC and 15 oC, respectively. The Mw was in the range of 500,000 to 820,000 Da, whereas the Mn was in the range of 235,000 to 340,000 Da with a Mw/Mn in the range of 2.1 to 2.5.

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